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Registro Completo |
Biblioteca(s): |
Embrapa Pesca e Aquicultura. |
Data corrente: |
28/04/2021 |
Data da última atualização: |
28/04/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CORNACINI, M. R.; MANOEL, R. O.; ALCANTARA, M. A. M.; MORAES, M. L. T.; SILVA, E. A. A.; PEREIRA NETO, L. G.; SEBBENN, A. M.; ROSSINI, B. C.; MARINO, C. L. |
Afiliação: |
MAIARA R. CORNACINI, UNESP, Botucatu-SP; RICARDO O. MANOEL, UNESP, Botucatu-SP; MARCELO A. M. ALCANTARA, UNESP, Botucatu-SP; MARIO L. T. MORAES, UNESP, Ilha Solteira-SP; EDVALDO A. A. SILVA, UNESP, Botucatu-SP; LEONEL GONCALVES PEREIRA NETO, CNPASA; ALEXANDRE M. SEBBENN, INSTITUTO FLORESTAL DE SÃO PAULO, Piracicaba-SP; BRUNO C. ROSSINI, UNESP, Botucatu-SP; CELSO L. MARINO, UNESP, Botucatu-SP. |
Título: |
Detection and application of novel SSR markers from transcriptome data for Astronium fraxinifolium Schott, a threatened Brazilian tree species. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Molecular Biology Reports, 2021. |
ISSN: |
0301-4851 |
DOI: |
https://doi.org/10.1007/s11033-021-06338-5 |
Idioma: |
Inglês |
Conteúdo: |
Astronium fraxinifolium is an endangered tree species from Brazil. Due to its significance in environmental reforestation, as well as the continued exploitation of its wood, it is necessary to develop management programs that support the conservation of the species. Simple sequence repeats (SSR) or microsatellite markers are widely used in population genetic studies across a range of diverse organisms. In this study, we present the first SSR markers developed for A. fraxinifolium as well as their frequency and distribution based on transcriptome data. From transcriptome data, we identified more than 100 thousand sequences presenting microsatellites, with a predominant distribution of trinucleotide repeats. From the initial screening, we selected 20 microsatellite loci which were validated and evaluated for genetic indices in two natural populations. All loci were polymorphic, ranging from four to 11 alleles per locus. The observed and expected heterozygosities ranged from 0 to 1.0 and from 0.533 to 1.0, respectively, while the genetic differentiation (GST = 0.363) was greater within than between populations. The developed SSR loci from RNA-Seq data provides a foundation for future studies on genetic diversity and population structure, mating system, and gene flow for A. fraxinifolium populations and related species, aiming at conservation and management. |
Palavras-Chave: |
Conservation genetics; Microsatellite markers. |
Thesagro: |
Anacardiaceae; Astronium Fraxinifolium. |
Thesaurus Nal: |
Conservation plants; Genetic markers; Microsatellite repeats; Population genetics. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/222904/1/mbr-2021.pdf
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Marc: |
LEADER 02452naa a2200337 a 4500 001 2131540 005 2021-04-28 008 2021 bl uuuu u00u1 u #d 022 $a0301-4851 024 7 $ahttps://doi.org/10.1007/s11033-021-06338-5$2DOI 100 1 $aCORNACINI, M. R. 245 $aDetection and application of novel SSR markers from transcriptome data for Astronium fraxinifolium Schott, a threatened Brazilian tree species.$h[electronic resource] 260 $c2021 520 $aAstronium fraxinifolium is an endangered tree species from Brazil. Due to its significance in environmental reforestation, as well as the continued exploitation of its wood, it is necessary to develop management programs that support the conservation of the species. Simple sequence repeats (SSR) or microsatellite markers are widely used in population genetic studies across a range of diverse organisms. In this study, we present the first SSR markers developed for A. fraxinifolium as well as their frequency and distribution based on transcriptome data. From transcriptome data, we identified more than 100 thousand sequences presenting microsatellites, with a predominant distribution of trinucleotide repeats. From the initial screening, we selected 20 microsatellite loci which were validated and evaluated for genetic indices in two natural populations. All loci were polymorphic, ranging from four to 11 alleles per locus. The observed and expected heterozygosities ranged from 0 to 1.0 and from 0.533 to 1.0, respectively, while the genetic differentiation (GST = 0.363) was greater within than between populations. The developed SSR loci from RNA-Seq data provides a foundation for future studies on genetic diversity and population structure, mating system, and gene flow for A. fraxinifolium populations and related species, aiming at conservation and management. 650 $aConservation plants 650 $aGenetic markers 650 $aMicrosatellite repeats 650 $aPopulation genetics 650 $aAnacardiaceae 650 $aAstronium Fraxinifolium 653 $aConservation genetics 653 $aMicrosatellite markers 700 1 $aMANOEL, R. O. 700 1 $aALCANTARA, M. A. M. 700 1 $aMORAES, M. L. T. 700 1 $aSILVA, E. A. A. 700 1 $aPEREIRA NETO, L. G. 700 1 $aSEBBENN, A. M. 700 1 $aROSSINI, B. C. 700 1 $aMARINO, C. L. 773 $tMolecular Biology Reports, 2021.
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Embrapa Pesca e Aquicultura (CNPASA) |
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Registro Completo
Biblioteca(s): |
Embrapa Clima Temperado. |
Data corrente: |
20/11/2014 |
Data da última atualização: |
20/11/2014 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
SCHEIBLER, R. B.; FIOREZE, V. I.; SOUZA, A. P. B. de; ROSA, P. P. da; SILVA, J. L. S. da; SCHAFHAUSER JUNIOR, J. |
Afiliação: |
RUDOLF BRAND SCHEIBLER, UFPEL; VICTOR IONATAN FIOREZE, UFPEL; ANA PAULA BINATO DE SOUZA, UFPEL; PATRICIA PINTO DA ROSA, UFPEL; JAMIR LUIS SILVA DA SILVA, CPACT; JORGE SCHAFHAUSER JUNIOR, CPACT. |
Título: |
Efeito da fonte energética do concentrado no perfil metabólico sanguíneo de vacas lactantes. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
In: CONGRESSO DE INICIAÇÃO CIENTÍFICA, 23.; ENCONTRO DE PÓS-GRADUAÇÃO, 16., 2014, Pelotas. [Anais.]. Pelotas: UFPel, 2014. |
Idioma: |
Português |
Palavras-Chave: |
Lactantes. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/112107/1/CA-01985.pdf
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Marc: |
LEADER 00631nam a2200169 a 4500 001 2000689 005 2014-11-20 008 2014 bl uuuu u00u1 u #d 100 1 $aSCHEIBLER, R. B. 245 $aEfeito da fonte energética do concentrado no perfil metabólico sanguíneo de vacas lactantes.$h[electronic resource] 260 $aIn: CONGRESSO DE INICIAÇÃO CIENTÍFICA, 23.; ENCONTRO DE PÓS-GRADUAÇÃO, 16., 2014, Pelotas. [Anais.]. Pelotas: UFPel$c2014 653 $aLactantes 700 1 $aFIOREZE, V. I. 700 1 $aSOUZA, A. P. B. de 700 1 $aROSA, P. P. da 700 1 $aSILVA, J. L. S. da 700 1 $aSCHAFHAUSER JUNIOR, J.
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